Ribosomal protein S12



Ribosomal protein S12 (RPS12) is one of the 22 proteins which belong to the small subunit of the bacterial ribosome. It is involved in the translation initiation step. The images at the left and at the right correspond to one representative RPS12, i.e. the crystal structure of Ribosomal protein S12 from Thermus thermophilus (2zm6, chain L).

3D Structures of Ribosomal protein S12
Update June 2011

RPS12
1qzc - TtRPS12+16S RRNA helix+23S RRNA helix - Thermus thermophilus

1pn7 – TtRPS12+TtRPL11+P-site tRNA+16S RRNA fragment+23S RRNA fragment

1pn8 - TtRPS12+TtRPL11+E-site tRNA+16S RRNA fragment+23S RRNA fragment

1mvr – EcRPS12+EcRPL11+mRNA codon+16S RRNA fragment+23S RRNA fragment – Cryo-EM - Escherichia coli

S12 in small ribosomal subunit
2zm6, 1n36, 1j5e, 3oto - TtRPS12 in 30S ribosomal subunit+THX+16S RRNA

2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq,  3pys,  3pyu - TtRPS12 in 30S ribosomal subunit+16S RRNA+RNA fragment

3kc4 – EcRPS12 in 30S ribosomal subunit

3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3or9, 3ora - EcRPS12 in 30S ribosomal subunit+16S RRNA

1i94, 1fka - TtRPS12 in 30S ribosomal subunit+16S RRNA

1p87, 1p6g - EcRPS12 in 30S ribosomal subunit+16S RRNA – Cryo-EM

3bbn - RPS12 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach

S12 in small + large ribosomal subunit
1ml5 - EcRPS12 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM

S12 in small ribosomal subunit+ chain release factor
3mr8, 3ms0 – TtRPS12 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA

3d5a, 3d5c - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA

2b64 - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1

2b9m - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2

2x9r, 2x9t, 2wh1, 2wh3 - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA

3f1e, 3f1g - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA

S12 in small ribosomal subunit+tRNA+mRNA
2hgi - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet

2hgp - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2i2p, 2i2u - EcRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2hgr - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA

1yl4 – TtRPS12 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator

1pns - EcRPS12 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA

1xnq, 1xnr - TtRPS12 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+A-site tRNA
2uxd, 2uxc - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA

1n32, 1n33 - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA

1n34 - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA

1xmo, 1xmq - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA

2uxb - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+P-site tRNA
3i8g - TtRPS12 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA

3i8h, 3i9b, 3i9d - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA

1ibk - TtRPS12 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA

2wwl - EcRPS12 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli

3i1q - EcRPS12 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA

3i1s, 3i1z, 3i21 - EcRPS12 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA

S12 in small ribosomal subunit+A-site tRNA+P-site tRNA
1ibl, 1ibm - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+P-site tRNA+E-site tRNA
2ow8 - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA

2b9o - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA

2qnh - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA

S12 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA
2gy9, 2gyb - EcRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM

1jgo, 1jgp, 1jgq, 1gix - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA

3e1a, 3e1c - EcRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA

S12 in small ribosomal subunit + antibiotics
1i95, 1i97, 2hhh,  2f4v, 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4  -  TtRPS12 in 30S ribosomal subunit+16S RRNA+antibiotic

1hnw, 1hnx, 1hnz,  1fjg - TtRPS12 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic

2qbb, 2qb9, 1vs5, 1vs7,  2qan,  2qal,  2qou,  2qow,  2qoy, 2qp0,  2z4k, 2z4m, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy - EcRPS12 in 30S ribosomal subunit+16S RRNA+antibiotic

2j00, 2j02 - TtRPS12 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA

2wdg, 2wdh, 2wdk, 2wdm - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe+paromomycin

3knj, 3knh - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin

3knl, 3knn - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin

S12 in small ribosomal subunit + ribosome recycling factor
2qbd, 2qbf – EcRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor

3r8n, 3r8o – EcRPS11 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA

2qbh, 2qbj - EcRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin

2v46, 2v48 - TtRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet

1zn1 - TtRPS12+16S RRNA fragment+23S RRNA fragment+ribosome recycling factor+ chain release factor

S12 in small ribosomal subunit + elongation factor
2wri, 2wrk - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G

2wrn, 2wrq - TtRPS12 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4

3fic - TtRPS12 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU-A

3izv – EcRPS12 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU

3izw – EcRPS12 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU

2y0u, 2y0w,  2y0y, 2y10,  2y12,  2y14,  2y16, 2y18 - TtRPS12 in 30S subunit+THX+A-site tRNA+E-site tRNA+mRNA codon+ 16S RRNA+elongation factor TU

3fih - EcRPS12 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU

3huw, 3huy - TtRPS12 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P

3ep2, 3eq3, 3eq4 – EcRPS12+EcRPL11+16S RRNA fragment+23S RRNA fragment+tRNA+elongation factor TU

2om7 - TtRPS12+TtRPL1+16S RRNA fragment+23S RRNA fragment+tRNA+elongation factor G

1mj1 – EcRPS12+EcRPS13+EcRPL11+16S RRNA fragment+23S RRNA fragment+tRNA Phe+elongation factor TU – Cryo-EM

S12 in small ribosomal subunit + translation initiation factor
1i96 - TtRPS12 in 30S ribosomal subunit+THX+IF3+16S RRNA

1hr0 - TtRPS12 in 30S ribosomal subunit+THX+IF+16S RRNA

S12 in small ribosomal subunit + various proteins
3kiq, 3kis, 3kiu, 3kix - TtRPS12 in 30S ribosomal subunit+toxin RelE+16S RRNA

1voq, 1vos, 1vov, 1vox, 1voz - EcRPS12 in 30S ribosomal subunit+protein Y+16S RRNA

2xfz, 2xg1 - TtRPS12 in 30S ribosomal subunit+tRNA Phe+mRNA+colicin E3+16S RRNA

3deg - EcRPS12+EcRPL11+16S RRNA fragment+23S RRNA fragment+A-site tRNA+P-site tRNA+LEPA EF4 fragment

3j00 – EcRPS12 + 16S RRNA + FTSQ + apolipoprotein A-I

2xzm, 2xzn - RPS12 in 40S subunit+18S RRNA+RACK1 Tetrahymena thermophila

S12 in part of small ribosomal subunit
3iy8 - EcRPS12+EcRPS5+EcRPS6+EcRPS9+EcRPS9 EcRPS8+EcRPS11+EcRPS15+EcRPS16+EcRPS17+EcRPS18+Leishmania taretolae mRNA

2r1g – TtRPS12+ TtRPS9+ TtRPS13+ 16S RRNA fragment